Glucagon ELISA – 10 Microliter

(10-1281-01)

Kit format: 1 x 96 wells

Measurement range: 2-180 pmol/L (7 - 627 pg/mL)

1.Low sample volume - 10 µL

2.No purification of samples

3.Highly appreciated and often cited assay

4.Controls available: 10-1286-01

5.Little or no cross-reactivity to oxyntomodulin, glicentin, mini-glucagon, GLP-1, GLP-2 and GRPP

DESCRIPTION

Mercodia Glucagon ELISA – 10 μL is a highly specific and sensitive immunoassay for measuring glucagon in serum, EDTA plasma, and cell culture media samples from rat, mouse, and non-human primates.

A revolutionizing ELISA for sensitive and specific determination of glucagon in rat, mouse, and non-human primate (NHP) samples. A research tool advancement that allows science to take the next step.

The highly specific monoclonal antibodies used in this assay enable accurate determination of glucagon with low to no levels of significant cross-reactivity to other circulating pro-glucagon-derived peptides. Under certain conditions like post-bariatric surgery, the concentrations of glicentin have been demonstrated to be elevated to levels that might interfere.

TEST CHARACTERISTICS

SPECIES：Rat, mouse and non-human primates (NHP)

SAMPLES：Serum, EDTA plasma or cell culture medium

SAMPLE VOLUME：10µL

ASSAY RANGE：2-180 pmol/L (7 - 627 pg/mL)

DETECTION LIMIT：≤1.5 µg/L)

INCUBATION：18-22 h (overnight) + 15 min

DETECTION METHOD：Absorbance

SPECIFICITY

The following cross-reactions have been tested:

MINI-GLUCAGON：Not detected

GLICENTIN, HUMAN：1.0 %

GLICENTIN, MOUSE：7.0 %

GLICENTIN, RAT：4.0 %

GLP-1 (7-36)：Not detected

GLP-1 (9-36)：Not detected

GLP-2：Not detected

OXYNTOMODULIN, HUMAN/RAT/MOUSE：2.0 %

OXYNTOMODULIN, BOVINE/CANINE/PORCINE：Not detected