Glicentin NL-ELISA

(10-1273-01)

Kit format: 1×96 wells

Measurement range: 3-300 pmol/L (24-2400 pg/mL)

1.Highly specific, sensitive and rapid method for determination of glicentin

2.No cross-reactivity to circulating proglucagon derived gut peptides

3.Assay buffers and calibrators have been optimized to prevent degradation and aggregation of glicentin

DESCRIPTION

The Mercodia Glicentin ELISA is a high quality immunoassay for the quantification of human glicentin in serum, plasma and cell culture media.

Glicentin is a proglucagon derived gut peptide that is released in the gut in response to feed intake. It is present in high levels in the circulation after stimulation and is often a problem when measuring glucagon and other proglucagon derivates because of it’s shared sequence. The Mercodia Glicentin ELISA however, has no cross-reactivity to such circulating proglucagon derived gut peptides.

TEST CHARACTERISTICS

SPECIES：Human

SAMPLE TYPES：Serum, EDTA plasma and cell culture media

SAMPLE VOLUME：25 µL

ASSAY RANGE：3 - 300 pmol/L (24-2400 pg/mL)

DETECTION LIMIT：≤3 pmol/L (24 pg/mL)

INCUBATION：1 h + 1 h + 15 min

DETECTION METHOD：Absorbance

SPECIFICITY

The following cross-reactions have been tested:

GLUCAGON：Not detected

OXYNTOMODULIN：Not detected

MINI-GLUCAGON：Not detected

GLP-1：Not detected

GLP-2：Not detected

RAT GLICENTIN：0.9 %

MOUSE GLICENTIN：Not detected