Insulin ELISA –10 pack

(10-1113-10)

Kit format: 1 x 96 wells

Measurement range: 3 - 200 mU/L (0.13 - 8.70 µg/L)

1.Calibrated against 1st International Reference Preparation 66/304 for human insulin

2.No cross-reactivity to C-peptide or Proinsulin des(31-32) or split (32-33)

3.Insignificant or no cross-reactivity to insulin analogs

4.CE/IVD labeled on select markets

5.Controls available: 10-1134-01 (US only) or 10-1164-01

DESCRIPTION

A high quality ELISA for specific measurement of human insulin in serum, plasma or cell culture media.

The Mercodia Insulin ELISAs are the only ELISA assays to satisfy the total error requirements in the Insulin Standardization Program .

TEST CHARACTERISTICS

SPECIES：Human

SAMPLES：Serum, plasma, cell culture medium

SAMPLE VOLUME：25µL

ASSAY RANGE：3 - 200 mU/L

DETECTION LIMIT：≤ 1 mU/L (0.043 µg/L)

INCUBATION：1h + 15 min

DETECTION METHOD：Absorbance

SPECIFICITY

The following cross-reactions have been tested:

C-PEPTIDE：<0.1 %

PROINSULIN：<0.01 %

PROINSULIN DES (31-32)：<0.5 %

PROINSULIN SPLIT (32-33)：<0.5 %

PROINSULIN DES (64-65)：98 %

PROINSULIN SPLIT (65-66)：56 %

INSULIN ASPART：4 %

INSULIN DETEMIR：Not detected

INSULIN GLARGIN：24%

INSULIN GLULISINE：Not detected

INSULIN LISPRO：Not detected

IGF-I：<0.02 %

IGF-II：<0.02 %

RAT INSULIN：0.7%

MOUSE INSULIN：0.3 %

PORCINE INSULIN：93 %

OVINE INSULIN：48 %

BOVINE INSULIN：31 %